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Viability, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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counting lite 3d luminescent cell viability assay kit - by Bioz Stars, 2026-07
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calcein pi cell viability cytotoxicity assay kit  (Beyotime)
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Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
Calcein Pi Cell Viability Cytotoxicity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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live dead viability cytotoxicity kit  (Fisher Scientific)
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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live dead cell viability assay kit  (Keygen Biotech)
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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Keygen Biotech calcein am pi cell viability cytotoxicity assay kit
Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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calcein am pi cell viability cytotoxicity assay kit - by Bioz Stars, 2026-07
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Image Search Results


Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

Journal: Bioactive Materials

Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

doi: 10.1016/j.bioactmat.2026.02.048

Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control

Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Journal: iScience

Article Title: Ready-to-use 3D bioprinted scaffolds from natural materials loaded with patient’s PRGF for personalized skin regeneration

doi: 10.1016/j.isci.2026.116185

Figure Lengend Snippet: Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: LIVE/DEAD® viability/cytotoxicity kit , Fisher Scientific , Cat# L3224.

Techniques: Staining, Imaging